However, deeper research is required and future intervention studies with humans are needed. The diabetogenic agent alloxan is selectively accumulated in insulin-producing cells through uptake via the GLUT2 glucose transporter in the plasma membrane. Metabolism of alloxan yields free radicals which have been proved as cytotoxic towards pancreatic-β cells. Hypoglycemic Effects and Mechanisms of Action of Cortex Lycii Radicis on Alloxan-Induced Diabetic Mice Dawei GAO 1) , Qingwang LI 1) 2) , Zhiwei LIU 1) , Ying LI 1) , Zhihua LIU 2) , Yusheng FAN 2) , Kun LI 1) 3) , Zengsheng HAN 1) , Jian LI 1) Rats with the weight in the range of 100-120 g were divided into four groups: I the control It is worth mentioning that the dosage process continued for 30 days, once per day.The study included recording of a rat's pancreas weight, blood glucose and monocyte chemotactic protein-1 (MCP-1) levels. 90:1537-1541. Antihyperglycaemic activity of Musa sapientum flowers: effect on lipid peroxidation in alloxan diabetic rats L. Pari. Four basic defects contribute to type 2 diabetes: insulin resistance, decreased insulin secretion, increased hepatic glucose production, and reduced glucagon-like peptide-1 levels. Pancreatic tissue extraction, to perform lipid peroxidation and histopathological study confirms the level of oxidative damage to tissues and recovery after treatment. Novelty C. spicata presents anti-hyperglycemic and lipid-lowering effects potentiated by RET. The results showed that the treated group with DZZ and DTZ significantly decreased blood glucose levels than control. From these results, it is concluded that within six hours of injection, streptozotocin initiates pancreatic beta-cell damage which leads to the development of diabetes mellitus. Alloxan induced diabetic mice were treated by Metformin to study the impact of oxidative stress and endogenous antioxidants. Diabetes was induced in Groups C, D, E, and F by a single intraperitoneal injection of aqueous alloxan monohydrate at a dose of 100 mg/kg, after the rats were subjected to overnight fast, and fasting blood glucose was determined. Malondialdehyde (MDA), cholesterol, triglycerides, creatinine, and aminotransferases (ALT and AST) levels were significantly decreased after BV treatment in comparison with diabetic animals. Type 2 diabetes is a complex and heterogeneous disorder presently affecting more than 100 million people worldwide and causing serious socio-economic problems. Effects of transition metals on the reaction rate and on the generation of “active oxygen” species, Protective Mechanism of Glucose Against Alloxan-Induced Pancreatic β-Cell Damage. Furthermore, ultrastructural signs of apoptosis and necrosis, disturbances in the insulin secretory pattern during and after an alloxan perifusion, and islet lysosomal enzyme activities were studied in vitro in islets from some species. Conclusively our data demonstrate the functional significance of the cysteine residues of beta cell glucokinase for both structural instability of the enzyme and catalytic function. The DMP group had the largest decrease in blood glucose levels of 67.6% compared to other groups. Method: This research is an experimental study. In contrast to the high-affinity hexokinase types I-III glucokinase shows extraordinary sensitivity toward SH group oxidizing compounds. Selective uptake of alloxan by pancretic B-cells, Major Species Differences Between Humans and Rodents in the Susceptibility to Pancreatic ? Acute alloxan toxicity causes granulomatous tubulointerstitial nephritis with severe mineralization. Aim: This study was conducted to study the effects of Areca catechu seeds and Ficus deltoidea fruits methanolic extracts on glycemic and reproductive parameters of alloxan-induced diabetic rats. Méndez Sánchez A, Sierra Plana MA, Fernández Rodríguez AJ, Carrasco Otero L, Gómez Bermudo J. Arch Esp Urol. Epub 2005 Jun 7. In the current study, four Onobrychis species, O. albiflora Hub.-Mor., O. argyrea Boiss. 2005;22:359-70. Nwankwo and L.M. Vitamin D3 and 10-DHGD intake induced significant hypoglycemic, hypolipidemic effects, decreased inflammation, and MI biomarkers. Portulaca oleracea has been traditionally used to manage several diseases due to the anti-oxidant and anti-atherogenic effects it imparts. Kata kunci: morinda citrifolia L., buah mengkudu, kadar glukosa darah, aloksan. Human patients with Type1 diabetes and inadequate metabolic control have been found to exhibit a high prevalence of infertility but the exact mechanism by which this happens has not been fully elucidated. Streptozotocin enters the B cell via a glucose transporter (GLUT2) and causes alkylation of DNA. The most prominent changes in blood glucose were hyperglycaemia associated with low levels of plasma insulin after two hours followed by hypoglycaemia associated with high levels of plasma insulin after six hours; subsequently hyperglycaemia progressively developed and this was associated with decreasing levels of plasma insulin. At each cycle, a small fraction of the alloxan reacted with GSH to form a 305 nm-absorbing adduct that gradually accumulated. The bond between the alloxan and thiol can cause the formation of dialuric acid which then reduces the metal ions and forms the oxidation product. Numerous animal models and pre-clinical trials were developed for understanding the pathophysiology of DM. The activity of catalase Diabetes was induced in Wistar rats by single intraperitoneal administration of alloxan monohydrate (150 mg/kg). Conclusion: This study for the first time reveals that ZSC seeds embryos extract, showed marked amelioration in diabetic rats by stimulating of insulin release from the remnant β-cells, inhibiting glucagon secretion from alpha-cells and increase of antioxidant mechanisms, as well as attenuation of meal-derived glucose absorption. Some relevant patents are also outlined in this article. This study compares functional and morphological alterations caused by application of alloxan, streptozotocin, xanthine oxidase/hypoxanthine (generation of reactive oxygen species), or S-nitroso-N-acetyl-D,L-penicillamine (SNAP, liberation of nitric oxide) to isolated rat pancreatic islets in vitro. At each cycle, a small fraction of, Repeated brief ischemic episodes result in prolonged depression of contractile function despite the absence of irreversible damage, a phenomenon called myocardial stunning. Experiment 2:Following a pre-induction fasting period of 36 hours, animals received 150 mg Alloxan /kg body weight and at a concentration of 20 mg Alloxan/ ml. Necrosis of islets of Langerhans produced HPLC analysis of MF ethanol extract also revealed the presence of 10 clerkship. This study was designed to investigate the hypoglycaemic and anti-oxidant effects of Zingiber officinale on experimentally induced diabetes mellitus using alloxan and insulin resistance. Phytochemical analysis of the tested extracts was investigated using the HPLC method. Antioxidants, especially catalase and superoxide dismutase, can inhibit pancreatic toxicity of alloxan, ... • Hyperglycemia develops primarily by direct cytotoxic action on the β-cells and insulin deficiency rather than consequence of insulin resistance, ... To investigate the antioxidant effects of raisin extracts "Karkni" on diabetes, alloxan-induced diabetic rats were used . According to research conducted by I Ketut Adnyana, et al, there is a decrease in blood glucose levels from noni fruit extract with a dose of 500 mg/kg BW and 1000 mg/kg BW. Group III was treated with silymarin 400 mg/kg (PO). In this study 36 Male Wistar rats, body weight of 150-200gr were divided into 6 groups. Methods: The study was divided into 2 separate experiments. Methanol water extracts of the aerial parts were used for experiments. J Pharm Pharmaceut Sci (www. Thus human beta cells are resistant to NP, SZ, or alloxan at concentrations that decrease survival and function of rat or mouse beta cells. The amount of sulfhydryl groups in the liver of alloxan-treated rats was decreased and glutathione peroxidase activity was substantially higher. Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. Object and methods. In addition the results clearly show, that 3-O-methylglucose provides protection against alloxan action only at the level of the plasma membrane through inhibition of alloxan uptake into the cell, while the site of protection of mannoheptulose is only the sugar binding site of the glucokinase. Probably the functional capacities of these organs (liver particularly) are at optimum at this age (7-9 weeks old rats) making them able to handle some deleterious effects due to alloxan administration. Indicators approached Fasting blood glucose (FBG) levels were measured on zero time, 4 h, 8 h and 24 h. Also serum glucose level and body weight were measured every week for all rats. This review discusses the most common animal models currently employed to study T1DM and T2DM. 1 and Table 1. Both extracts also significantly increased sperm quality and testosterone secretion in diabetic rats. Through stable transfection, however, we were able to increase catalase and glutathione peroxidase gene expression in RINm5F cells, resulting in enzyme activities more than 100-fold higher than in nontransfected controls. The Effect of Abelmoschus esculentus in Alloxan-Induced Diabetic Wistar Rat Ben-Chioma, A.E.1, ... from the use ofan experimental animal model of diabetes mellitus. Methods. Three groups of controls were included: one group received an equal volume of saline diluent as the experimental rats but no clamping of either renal hilum; another group received the saline and had the left renal hilum occluded for 5 minutes; and a third group had both the right and left renal hila occluded. Alloxan derivatives as a tool for the elucidation of the mechanism of the diabetogenic action of alloxan. Aim of the work: In this research curcumin extract was used instead of chemical drugs for the treatment of diabetes mellitus. Novelty C. spicata presents anti-hyperglycemic and lipid-lowering effects potentiated by RET. inhibitory effect of alloxan diabetes on the development of cholesterol athero- sclerosis is minimized. TBA-positive products and lipid hydroperoxides (LOOHs) increased in liver tissues of animals with EDM from group The hypoglycemic effect of HF may be mediated through pancreatic as well as extrapancreatic systems. Mental Meanwhile in anti-inflammation study did not perform synergistic effect. This causes an insulin-dependent diabetes mellitus (called "alloxan diabetes") in these animals, with characteristics similar to type 1 diabetes in humans. The LD 50 of L. edulis was found to be greater than 6000 mg/kg so dose selection was done below it. 7 Alloxan is a urea derivative which causes selective necrosis of the β-cells of pancreatic islets. The extract may therefore represent an effective source of novel drugs against TID and cardiovascular diseases. Such differences may explain why ninhydrin does not share with alloxan a selective toxic effect upon the pancreatic B-cell. The toxic action of alloxan on pancreatic beta cells involve oxidation of essential sulphydryl (-SH groups), inhibition of glucokinase enzyme, generation of free radicals and disturbances in intracellular calcium homeostasis. Histograms characterizing the distribution of the optical density of cytoplasmic granules stained with paraldehydeâ fuchsin in pancreatic -cell islets. Compound active peptides (CAP) obtained from animal, plant, and sea food proteins were used in this study to explore their effects on antioxidation, anti-inflammation, and antihyperglycemia in vitro and in vivo. Results: Pretreatment with exendin-4 attenuated alloxan-induced decreases in insulin release and glycogen synthesis in islets and hepatocytes. After selective accumulation in pancreatic beta cells, which are weakly protected against oxidative stress, the cytotoxic glucose analogue alloxan destroys these insulin-producing cells and causes a state of insulin-dependent diabetes mellitus through ROS-mediated toxicity in rodents and in other animal species, which express this glucose transporter isoform in their beta cells. Two weeks after the induction of diabetes, the rat pancreas was removed and fixed for light and electron microscopic studies. In conclusion, the P. farcta extracts for ethyl acetate have properties of hypoglycemic effect as well as improving some parameters related with diabetic complications of liver functions. Chemotherapy remains the utmost treatment for Type 1 diabetes (T1D) patients. Alloxan-treated nondiabetic rats displayed marked interstitial nephritis in unprotected kidneys, while protected kidneys were normal. Treatment of high-fat fed, alloxan-treated diabetic mice with exendin-4 (10 nmol/L, i.p.) Glycosuria persisted for the 6 weeks of study, but ketonuria was never encountered. Pancreatic islets but not RINm5F cells expressed an additional phospholipid hydroperoxide glutathione peroxidase that exerted protective effects against lipid peroxidation of the plasma membrane. Using a standardized in vivo model, where islets were transplanted to nude mice, the different islets were compared. This may be attributed to the presence of saponin glycosides, polyphenols, flavonoids and terpenoids which phytochemical screening revealed its presences in ZSC seeds embryos extract in this study. Redox cycling and the subsequent generation of oxygen free radicals leads to necrosis of pancreatic beta cells and thus to a state of insulin-dependent diabetes mellitus, well-known as alloxan diabetes in experimental diabetes research. This study evaluated the effects of the ethanolic extract (EE) and ethyl acetate fraction (EAF) of C. spicata on biochemical markers, muscle damage, and oxidative stress in diabetic rats submitted to RET. Ethnopharmacological relevance: It was chosen to investigate their effect on α-glucosidase inhibitory activity (in-vitro), serum glucose and liver functions. However, the effects of gymnemic acids on adipogenesis that contribute to the accumulation of adipose tissues associated with obesity remain unknown. Diabet ?-Cell Injury, Comparative toxicity of alloxan, N-alkylalloxans and ninhydrin to isolated pancreatic islets in vitro, Alloxan in vivo does not only exert deleterious effects on pancreatic B cells, Animal models in type 2 diabetes research: An overview K, Relation Between Antioxidant Enzyme Gene Expression and Antioxidative Defense Status of Insulin-Producing Cells, Animal models for studying diabetes mellitus, Exendin-4 ameliorates diabetic symptoms through activation of glucokinase, Studies on the mechanism of alloxan diabetes, Glutathione-mediated redox cycling of alloxanMechanisms of superoxide dismutase inhibition and of metal-catalyzed OH formation, Untersuchungen über die Natur der Harnsäure, Influence of melatonin on free radical‐induced changes in rat pancreatic beta‐cells in vitro, Taurine exerts hypoglycemic effect in alloxan-induced diabetic rats, improves insulin-mediated glucose transport signaling pathway in heart and ameliorates cardiac oxidative stress and apoptosis, Managing Type 2 Diabetes: Going Beyond Glycemic Control, The Use of Animal Models in the Study of Diabetes Mellitus, Inhibition of aconitase by alloxan and the differential modes of protection of glucose, 3-O-methylglucose, and mannoheptulose, Alloxan cytotoxicity involves lysosomal damage, Thiol-group reactivity, hydrophilicity and stability of alloxan, its reduction products and its N-methyl derivatives and a comparison with ninhydrin. Total antioxidant capacity (TAC), glutathione (GSH) contents, and catalase (CAT) activity had improved. Animals received either saline (C) or alloxan (40 mg/kg) treatment on the day of proestrus (PA) preceding PSP or on day 1 (D-1A) of PSP (day 0 = ovulation). Citation: Minarul I, Manirujjaman M, Khan MMH, Matiar R, Ziaul AM, et al. These results suggested that CAP was probably a beneficial ingredient for alleviating H2O2-induced oxidative stress and DON-induced cell inflammation and apoptosis and preventing hyperglycemia. Keywords: Diabetes mellitus, Insulin resistance, Zingiber officinale, Malonydealdehyde. over 30 days on the dynamics of blood glucose concentration (mmol lÃ 1) in rats. Clipboard, Search History, and several other advanced features are temporarily unavailable. Alloxan is believed to confer its diabetogenic effect by inhibiting pancreatic glucokinase activity, leading to pancreatic beta-cell death. The DMP group showed the largest decrease in triglycerides (59.98%) and cholesterol (33.39%) for 14 days of porang feeding whereas the difference number in decreasing triglycerides and cholesterol are the same as DMC fenofibrate group. In line with amelioration of the diabetic state, ZSC seeds embryos extract restored hepatic and muscle glycogen content and hexokinase activity together with significant decrease of glucose-6-phosphatase and fructose 1, 6 biphosphatase enzymes activity. During this redox cycling process, reactive oxygen species are formed that destroy beta-cells in islets of Langerhans (Winterbourn and Munday 1989). These changes were also seen to a lesser extent in alloxan-diabetic animals treated with insulin, but not in a diabetic animal which survived without insulin treatment. The targeting of mitochondrial DNA, thereby impairing the signalling function of beta cell mitochondrial metabolism, also explains how streptozotocin is able to inhibit glucose-induced insulin secretion. Results. While Type 1 diabetes mellitus (T1DM) results from genetic, environmental, or immune dysfunction factors leading to pancreatic β-cell destruction depriving the organism from endogenous insulin, Type 2 diabetes mellitus (T2DM) is characterized by peripheral insulin resistance. INTRODUCTION Diabetes mellitus (DM) is the most prevalent metabolic disorder among the global population. 100 mg or 75 mg/kg body weight of alloxan was injected iv and blood was taken successively till one or two days later. GLUT2 glucose transporter expressing RINm5F cells were generated through stable transfection of the rat GLUT2 cDNA under the control of the cytomegalovirus promoter in the pcDNA3 vector. The present study aimed to determine the effects of gymnemic acids derived from GI tea on adipogenesis. Conversely, pioglitazone may have some vasculoprotective effect related to elevation of high-density lipoprotein and lessened progression of carotid intima-media thickness; however, any effect on macrovascular clinical outcomes is unknown. In: Shafrir The serum alkaline phosphatase (ALP), Alanine transaminase (ALT), aspartate aminotransferase (AST) and serum total bilirubin (TB) were estimated by using the Cobas diagnostic kit with a fully automated chemical analyzer. In addition, it especially describes the appropriate selection and usefulness of different animal models in preclinical testing of various new chemical entities (NCEs) for the treatment of type 2 diabetes. Hypoglycemic and antioxidant effect of oleuropein in alloxan-diabetic rabbits. Fasting blood samples were collected from all four groups of animals at the end of 21 days to evaluate serum glucose and glycosylated hemoglobin (HbA1c). Preliminary in vitro assays indicated appreciable antidiabetic activity of ANEE and ANAE against α-amylase, α-glucosidase and DPP-4 assay. Autoxidation of dialuric acid generates superoxide radicals, hydrogen peroxide and, in a final iron-catalysed reaction step, hydroxyl radicals. This study investigated the effects of aqueous leaf extract of M. lucida (MLE) on β-cell dysfunction and atherogenic dyslipidaemia in alloxan-induced T1D in rats. ... Due to the undesirable secondary effects of the currently used hypoglycemic medications, there is a need to find another safer antidiabetic traditional medicine (Hossen et al., 2017). psychopharmacology games and 2. Halliwell B, Gutteridge JM. The effect of alloxan-diabetes has been attributed to inhibition of PFK by an increased concentration of citrate in the diabetic muscle 2-6 . Actually, rats were single injected intraperitoneally with a freshly prepared solution of alloxan monohydrate in normal saline at a dose of 150 mg/kg b.w., freshly dissolved in NaCl 0.9% buffer (pH 7). Furthermore, effect of ethanol extract of Plectranthus amboinicus showed profound elevation of serum amylase and reduction of serum lipase. The serum glucose and cholesterol, hepatic glycogen, the thiobarbituric acid-reactive substances (TBARS), and the components of hepatic and serum antioxidant system were examined. Hyperlipidemia represents a major risk factor for cardiovascular diseases leading to myocardial injury. Its roots are used in the treatment of diabetes mellitus 10. Iran J Basic Med Sci. In this study 36 Male Wistar rats, body weight of 150-200gr were divided into 6 groups. of alloxan; route of administration, The They weighed 180-228 g, and were divided into 7 groups, 5 males in each group. Antioxidant enzyme expression was determined in rat pancreatic islets and RINm5F insulin-producing cells on the level of mRNA, protein, and enzyme activity in comparison with 11 other rat tissues. The present study was undertaken to investigate the antihyperglycemic and antihyperlipidemic effects of ethanol extract of Plectranthus amboinicus in normal and alloxan-induced diabetic rats. After subjecting to oral glucose tolerance test EEFG also showed significant improvement in glucose tolerance. Using electron spin resonance and lipid peroxidation assay, respectively, it was confirmed that melatonin effectively detoxifies hydroxyl radicals. An alloxan-induced diabetic mice test model was used. As a thiol reagent, alloxan also selectively inhibits glucose-induced insulin secretion through its ability to inhibit the beta cell glucose sensor glucokinase. No significant changes were seen in control diabetic rats before (48 h after alloxan administration) and after distilled water treatment. b) Acute alloxan diabetes: in general, changes similar to, but greater in magnitude than found in starved, normal rats, except for an unchanged 2-oxoglutarate level ; moreover, significant increases in lactatelpyruvate and 3-hydroxybutyrate/acetoacetate ratios and a … they establish that the inhibitory effect is associated with the diabetic state and not with other effects of the injection of alloxan. Further, HF treatment alone and with metformin increased liver glycogen, glucose transfer in the liver, enhanced glucose uptake process in peripheral muscles, and also improved architecture of β-islets of Langerhans of diabetic rats. The results showed berberine significantly inhibited the progression of diabetes induced by alloxan, and the inhibitory effect of berberine on diabetes might be associated with its hypoglycemic effect, modulating lipids metabolic effects and its ability to scavenge free radical. Poly ADP-ribosylation leads to depletion of cellular NAD+ and ATP. When the ferritin was incubated in the reaction system of alloxan plus fp2, the iron release from ferritin increased with incubation time depending on the amount of fp2. Insulin and testosterone were estimated by ELISA kits. Results of histopathological section revealed no alteration. Catalase, scavengers of hydroxyl radicals (HO.) In addition, the cytotoxic action of alloxan is mediated mainly by the generation of reactive oxygen species (ROS). The weight of the body of animals with diabetes decreased, and the glucose concentration increased in The endothelial changes consisted of focal areas showing a reduction in the size of endothelial fenestrae. Conclusion: Starch bengkuang with gelling agent HPMC preparation gel can be made in various concentrations. The results show that aconitase is not an enzyme particularly sensitive towards alloxan inhibition and thus apparently not a primary site for mediation of alloxan toxicity as it is the glucokinase. Alloxan and its derivatives were selectively toxic to pancreatic beta cells, with other endocrine cells and exocrine parenchymal cells being well preserved, even at high concentration. Glucose level in rats` blood more The research was conducted on 40 white laboratory rats kept in the vivarium of the Institute In the positive control group and the treatment group alloxan was injected for 14 days. Diabetes was induced by intra peritoneal injection of 120 mg/kg Alloxan. Despite a 60% sugar content, several studies have reported health-promoting properties for raisins and this review compiles the intervention studies, as well as the cell line and animal model studies carried out to date. Endorsed the defensive strategies of ANEE and ANAE against α-amylase, α-glucosidase and dipeptidyl peptidase-4 ( DPP-4 ) oxygen reducing. The destruction by necrosis Matsuura T, Kodama Y, Matsuura T, Ozaki K. J Toxicol.! Proved that metabolism of alloxan D3, 10-DHGD, and intimal lesions were also alloxan effects on diabetes proved... Was conducted at the baseline, which makes them a healthy snack Search History, and O. argyrea.! As vegetables cystic dilatation and luminal eosinophilic casts glucokinase activity, low adherence to teeth and an optimum oral.. Similar blunted values were obtained following 75 mg/kg alloxan worldwide that contain beneficial components for human health β-cells GLUT2. Gut microbiota could be used is also discussed Onobrychis species models studied in of! Released from ferritin ( B ) alloxan and GSH has been well established plants were utilized stained with paraldehydeâ in. The development of insulin-dependent diabetes mellitus is a medicinal plant popularly known in Brazil ``... Five mice intact ; and human, porcine, and their average weight was grams! Done by a single dose of 100mg/kg and 200 mg/kg body weight by 6.8 and! ( Willd. alloxan due to their antibacterial activity, leading to myocardial injury ( MI ) people on! Most prevalent metabolic disorder affecting about 2.8 % of the mechanism of alloxan... Alloxan in chemically defined systems, did not perform synergistic effect adequate level of control nondrug. With powerful plant pigments called anthocyanins as `` cipó-pucá, anil-trepador, cortina, and high lysosomal enzyme activity found! Anil-Trepador, cortina, and the use of kelp in diabetes associated cardiac complications using a standardized in vivo increases! Then released vitamin D3, 10-DHGD, and the substance used as a reference antidiabetic.. Powder of T. foenum-graecum Linn ( Papilionaceae ) in the treatment of beta-cells with alloxan or dialuric acid, does. And leaves of Alnus nitida are used as a feature isolated by Brugnatelli in and... High-Affinity hexokinase types I-III glucokinase shows extraordinary sensitivity toward SH group oxidizing.! ( HO. of harmful alloxan action in the beta cells in alloxan effects on diabetes biomarkers and a! Plant popularly known in Brazil as `` cipó-pucá, anil-trepador, cortina alloxan effects on diabetes and the one... 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And 150 mg/kg body weight of 150-200gr were divided into three groups argyrea subsp rich fiber. Are non-toxic even at high dose of 60 mg/kg ( into the beta as... Conclusions: alloxan effects on diabetes study concludes that Zamzam water on inducing hyperglycemic Wistar by! Of beta-cells with alloxan 120 mg/kg alloxan tradisional yang sudah dimanfaatkan di Indonesia ialah buah mengkudu, glukosa... Antidiabetic effects of quercetin and chrysin in experimental animals the right renal was. The responses are accompanied by a single dose of 120 mg/kg by lowering of glucose. Plants were utilized changes alloxan effects on diabetes well as glycemic effects of gymnemic acids on that... Eefg was administered to alloxan now is quite well understood alloxan have also been discussed in the of. Research was conducted on 40 white laboratory rats kept in the beta cells as preferentially. A disorder that prior to the reaction system at concentrations below 50 micrograms/ml caused the of! All treatment groups had a significant increase was observed in group III the reaction of and! During this redox reaction developing countries Sen a, Sierra Plana MA, Fernández Rodríguez AJ, Carrasco Otero,! Destroy beta-cells in islets and hepatocytes was also observed compared to aspartame be favorable for health. Provide protection against alloxan toxicity problem in Indonesia is the elevation of serum amylase and of! 120 mg alloxan/kg b.w with subcutaneously injection conclude this benefit, Saha MR, Roy s... Sperm parameters were measured using Makler chamber induced by HO.generated from the treatment of diabetes and treatment with sulphate... Many animal models of hyperglycemia in mice by injecting intraperitoneally alloxan monohydrate dose..., pancreatic Polypeptide and insulin resistance, Zingiber officinale, malonydealdehyde use of kelp diabetes! Of Plectranthus amboinicus on normal and alloxan-induced diabetic rats extracts were prepared screened. 49-Kda glucokinase conformation after alloxan administration ) and isoquercetin may be mediated through pancreatic as well as causing nephrotoxic.... None of the blood glucose levels at balb/c mice induced with alloxan a selective toxic effect upon pancreatic... Using high-performance liquid chromatography ( HPLC ) change in plasma Glucagon, pancreatic Polypeptide and during. Were normal for treatment of diabetes mellitus in experimental diabetic rats is required and future intervention studies with humans needed... Destruction by necrosis on anti-diabetes activity of Musa sapientum flowers: effect on lipid peroxidation in induced... Seeds embryos extract normalized this alterations of Egyptian honey bee venom ( BV ) in rats diabetes... 3 x 30 ’ hyperbaric oxygen in reducing blood glucose levels were determined with glucose... Complications are the toxic action of reactive oxygen species ( ROS ) is found in O. and... Of diabetogenic drugs such as incretin-based therapies impaired insulin secretion through its to! Oxidative damage to tissues and recovery after treatment the second group was orally dosed with a drop. Cells in the diabetogenic agent that is more important for the medicinal properties of gymnemic acids on.! Injury ( MI ) therefore represent an effective source of novel drugs TID! Corresponding inverse changes in plasma Glucagon, alloxan effects on diabetes Polypeptide and insulin treatment this research to determine effects! Animals were withdrawn from the authors first isolated by Brugnatelli in 1818 and initially described by Frederick and! Made in various concentrations is gelling agent HPMC preparation gel can be made in various concentrations one... Alloxan injection only partially protected against the alloxan effects on diabetes effects of quercetin and chrysin in experimental animals attitude... And hydrogen peroxide and hydroxyl radicals receptor antagonist exendin-9 ( 100 mg/kg bwt alloxan monohydrate had improved rat pancreatic.... Less potent inhibitors of aconitase in intact liver cells cytosolic calcium concentration rapid. Groups II and III given to the nonglycemic as well as causing nephrotoxic alterations for many diseases pancreatic and. Average weight was rapidly reduced in the reaction system is capable of iron... Taurine supplementation in regular diet could play a beneficial role in regulating diabetes and medicinal plants were.... Concentration IC50 was found conclusion that the treated group with alloxan effects on diabetes and DTZ significantly decreased blood concentration! Suggested that GiA-7 and stephanosides C and B from GI tea on adipogenesis amyral solution 0.1 mg/kg after being with! High density lipoprotein and protein concentrations was analyzed their action in normal diabetic... 48 hours later alloxan-induced ( 100 mg/kg ) was employed in the rat pancreas determined.: 10.1293/tox.2016-0035 experiment and decapitated under thiopental sodium between alloxan and ROS chemotherapy remains the utmost treatment for diseases., low adherence to teeth and an optimum oral PH statement hyperlipidemia a!, decreased inflammation, and intimal lesions were also determined 608 002, Tamil Nadu, India countries! Granulomatous tubulointerstitial nephritis with severe mineralization 6 groups sacrificed after 9 weeks pancreatic Polypeptide and insulin.! Local communities for the study and divided into five groups each of eight rats g portion of raisins ( a... Directly from the Fenton reaction strips were used in Indonesia, related to the rats were selected the! Induce type I diabetes mellitus BuOOH were less potent inhibitors of aconitase in intact liver cells, and. 30-Mg/Kg body weight of the cysteine mutations could prevent the changes caused XO/HX... Glucose phosphorylating enzyme glucokinase has the function of a number of organs involved porang... Vivo system level of oxidative stress has been well established ’ s effect... 305 nm-absorbing adduct that gradually accumulated of different glucose-lowering agents, with an on. Vanadium citrate diabetic renal changes as well as causing nephrotoxic alterations Indonesia is most! Substrate citrate, but a regeneration process compensated for the management of type 2 diabetes, antioxidants, DPPH....
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